Titre : Efficacy and environmental acceptability of two ballast water treatment chemicals and an alkylamine based-biocide
Année : 2010
Auteur(s) : La Carbona S. Viitasalo-Frösen S. Masson D. Sassi J. Pineau S. Lehtiniemi M. Corroler D.
Abstract : Ship's ballast waters transport large numbers of organisms which may become invasive in coastal regions.
One option to address this problem is the use of biocides as ballast water treatment (BWT). Ef?cacy and
environmental acceptability of three commercial active substances (the BWT biocides Peraclean® Ocean and
Seakleen®, and alkylamine-based biocide Mexel® 432/336) were tested against three bacteria species, two
vegetative microalgae and one zooplanktonic larva, in 10 and 30 Practical Salinity Unit (PSU) waters. In both
salinities, PeraClean® Ocean was the most effective biocide against bacteria causing N 90% mortality at 20 mg/l,
compared with 50 mg/l for Mexel® 432/336 and N 500 mg/l for Seakleen®. Regarding zooplankton, Seakleen®
was the most effective chemical causing 90% mortality in 24 h at concentrations b 6 mg/l (LC9024 h) in both
salinities, compared with 23 and 26 mg/l for Mexel® 432/336 and 370 and 480 mg/l for PeraClean® Ocean in
10 and 30 PSU, respectively. Similar pattern of ef?cacy was obtained for microalgae in 30 PSU: effective
concentrations inducing 50% growth inhibition in 4 days were ? 1.6 mg/l for Seakleen®, ? 10.1 mg/l for
Mexel® 432/336 and ?30.9 mg/l for PeraClean® Ocean. Our work highlighted that treated waters displayed
residual toxicity after 24 h still inducing mortality depending on the organism and biocide. However Mexel®
432/336 is the only biocide which had no impact on oyster larvae development at effective concentration.
Altogether our data showed that Mexel® 432/336 was the only biocide displaying a broad spectrum ef?cacy in
concentrations b 50 mg/l and not toxic for oyster larvae development at this concentration. However residual
toxicity of treated waters for any organism should be taken into account in BWT systems utilising biocides.
STOTEN-12286; No of Pages 9
Titre : Dynamics of bacterial consortia and Sulphate-Reducing Microorganisms associated to ALWC products in marine port structures
Année : 2010
Auteur(s) : Paisse S. Ghiglione JF. Gueune H. Marty F. Muyzer G. Quillet L.
Abstract : European port authorities are commonly concerned by Accelerated Low Water Corrosion (ALWC) occurring in low water zone of
steel port structures. High corrosion rates induced can shorten drastically service life of the structure or lead to failure. Solutions to
mitigate this corrosion are available but still remains costly. The goal of the MICSIPE project (Microbiologically Induced Corrosion of
Steel structures In Port Environment) is to develop risk assessment methods to predict and assess ALWC for industrial end users,
combining microbiological, chemical and corrosion sciences approaches.
Chemical process of corrosion has been already well documented. ALWC is microbiologically induced; but mechanisms and
bacteria involved have not been entirely determined until now. In this context, we studied the abundance, activity and diversity of bacteria
and particularly Sulphate-Reducing Microorganisms (SRM) in both suspected accelerated (ALWC) and normal (NLWC) low water
corrosion products from a European port.
ISME 13 – Microbes - Stewards of a changing planet – 22-27 August 2010 - Seattle, WA, USA
Titre : Biocorrosion – Corrosion Influencée ou Induite par les Micro-organismes
Année : 2010
Auteur(s) : Antony H. Gueune H. Poisson A.
Abstract : A study performed in 1990-1993 estimated a minimum of 12-13% of European
harbours affected by a particularly aggressive localized MIC phenomenon, called
Accelerated Low Water Corrosion (ALWC) occurring at or below the low water zone
of steel waterfront structures. ALWC is well recognized to affect harbours worldwide,
causing drastic reductions of the service life of steel structures due to unusual high
corrosion rates. Prevention and mitigation solutions exist, but are costly, while ALWC
prediction has so far not been possible due to the lack of understanding of abiotic
and biotic factors and of the mechanisms involved. Presence and potential
implication of sulfate-reducing and other sulfur bacteria in ALWC has been reported
in previous studies. However, the mechanisms and the characteristics of the biofilm-
deposit complex involved in ALWC remain unclear.
The objective of this study is to obtain a detailed characterization of ALWC biofilm-
deposit complexes taken from one European harbour in order to propose interaction
and corrosion mechanisms.
Several samples of corroded and non-corroded zones were recently taken from a
European harbour affected by ALWC since 20 years. These samples were studied
using different analytical methods: (1) cultivation targeting for the first time almost all
MIC associated-bacterial metabolic groups (sulfate-reducing bacteria (SRB),
thiosulfate-reducing bacteria (TRB), thiosulfate-oxidizing bacteria (TOB), iron-
oxidizing bacteria (IOB), iron-reducing bacteria (IRB), manganese-reducing bacteria
(MnRB) and marine aerobic heterotrophic bacteria); (2) 16S rDNA PCR-DGGE
fingerprinting methods, (3) microscopic methods (e.g. SEM) and (4) analytical
methods to determine chemical composition (i.e. XRD, XRFS, and Raman
spectrometry).
All targeted bacterial groups could be enriched from samples originating from ALWC
and from the reference sites confirming that the presence of specific metabolic
groups is not an exclusive criterion for characterizing ALWC biofilm. However, IOB
activities appeared to be higher in ALWC than in reference samples indicating a
potential implication of this metabolic group, which was not yet considered in ALWC.
Analysis of SRB and TRB enrichments indicated specific populations associated with
ALWC.
Analysis of the different layers of biofilm-deposit complexes indicated the presence of
an oxygen gradient within the biofilm/deposit complexes. Green rust and iron sulfide
detected with SRB in the inner layers of ALWC samples indicated the occurrence of
SRB activities.
Further results and their interpretation will be discussed in the future to improve
ALWC diagnostics and prediction.
The work was carried out with a financial grant from the Research Fund for Coal and
Steel of the European Community (Project number: RFSR-CT-2008-00018).
EUROCORR - Journée de l’Institut de soudure Date : 18 juin 2010
Titre : Aluminium accumulation in mussel Mytilus edulis: laboratory investigation
Année : 2009
Auteur(s) : Mao A. Caplat Ch. Mahaut ML. Pineau S. Barillier D.
Abstract : Non disponible
SETAC Europe 19th Annual Meeting, 31 May – 4 June 2009, Götegorg, Sweden
Titre : Comportement des aciers inoxydables exposés en milieu marin
Année : 2009
Auteur(s) : Antony H.
Abstract : Non disponible
lundi de la mécanique - CETIM
Titre : Installation véhiculant ou stockant de l'eau - Matériaux & Corrosion
Année : 2009
Auteur(s) : Antony H.
Abstract : Non disponible
USIDO
Titre : Formation of the Fe(II-III) hydroxysulphate green rust during marine corrosion of steel associated to molecular detection of dissimilatory sulfite-reductase
Année : 2008
Auteur(s) : Pineau S. Sabot R. Quillet L. Jeannin M. Caplat Ch. Dupont-Morral I. Refait Ph.
Titre : Process and Efficiency of In Situ Experimentation for Accelerated Corrosion Studies, in French Marine Ports
Année : 2008
Auteur(s) : Pineau S. Lefèvre Y. Dupont-Morral I. Marsset B. Scherrer P. Auzeby C. Bénaïssa B.
Abstract : Non disponible
Material Performance, October 2008, p. 2-6
Titre : The study of relationship between diversity of bacterial biofilms and marine corrosion of carbon steel in harbour environments
Année : 2008
Auteur(s) : Pineau S. Ghiglione JF. Quillet L. Caplat Ch. Refait Ph.
Abstract : Here is reported investigation aimed to elucidate relationship between accelerated marine corrosion of carbon steel and community structure of bacterial biofilms associated with corrosion products. The study was conducted in three different French coastal regions (English Channel, Atlantic Ocean and Mediterranean Sea). SEM-EDX, ICP-AES, XRD and micro-Raman spectrometry revealed stratification of corrosion products based on iron oxidation state, regardless of geographical location. Molecular biology techniques and standard cultivation methods demonstrated that bacteria in corrosion products were abundant and highly diverse. Moreover, corrosion products contained many species of diatoms and multicellular organisms. Characterisation of cultivable bacteria and culture-independent molecular fingerprinting method (CE-SSCP) revealed more than 20 bacterial ribotypes in the deposits. Molecular detection of sulphate-reducing bacteria (SRB) was carried out using dissimilatory sulfite-reductase gene (dsrAB) and 16S rDNA probes specific for six SRB sub-groups.
The results of microbiological analysis demonstrated that the composition and diversity of bacterial populations associated with corrosion products are determined by the chemistry of different regions, i.e. steel surface, corrosion product surface and green rust / oxy-hydroxide interface, without clear stratification. Moreover, the study confirmed that, irrespective of the site investigated, bacterial species detected in corrosion products differed from the ones present in bulk water, thus emphasising the importance of biofilm sampling when investigating corrosion failures.
In view of the obtained results, the role of green rust as sulphate reservoir, the localised acidification induced by biogenically produced sulphur, the plausible role of extracellular polymeric substances in connecting bacterial cells and mineral corrosion products and the impact of multicellular organisms on the corrosion product stability are discussed.
14th International Congress on Marine Corrosion and Fouling July 27-31, 2008 Kobe, Japan
Titre : Biofilm et matériaux : je t’aime moi non plus ». XIème colloque du Groupe Français de Bioélectrochimie
Année : 2008
Auteur(s) : Pineau S.
Abstract : A une époque où la tendance serait à la stérilisation généralisée, les biofilms sont souvent une source de problèmes sanitaires et industriels, liés à des effets toxiques mais également à des impacts sur les infrastructures (modification des propriétés de surface ou corrosion par exemple). Ces communautés biologiques, de l’échelle micrométrique à centimétrique selon la thématique (biofilms bactériens ou évolution en macro-biofilms pluricellulaires), présentent une affinité toute particulière pour la plupart des matériaux, métalliques ou non. Il s’agit donc d’un processus normal, naturel et généralement bénéfique d’un point de vue écologique. Néanmoins, bien que les matériaux soient conçus pour résister à un environnement donné, les micro-organismes viennent fréquemment perturber le sommeil des gestionnaires de ces structures. Difficiles à modéliser, à prédire et à maîtriser, que ce soit pour l’expérimentation scientifique ou pour le suivi industriel, ces interactions entre biofilms et matériaux concernent tous les domaines de compétences mais principalement la science des matériaux, la microbiologie et l’électrochimie. Cette communication pluridisciplinaire, parfois laborieuse, offre de nombreuses perspectives de recherche. Une partie de cette problématique est illustrée par des exemples issus de cas industriels, particulièrement en eau de mer, où la distinction des frontières minéral/organique et bénéfique/préjudiciable est discutée.
